Validation of reference genes for gene expression studies in the honey bee,Apis mellifera, by quantitative real-time RT-PCR
نویسندگان
چکیده
منابع مشابه
Selection and Validation of Reference Genes for Gene Expression Analysis in Vigna angularis Using Quantitative Real-Time RT-PCR
Adzuki bean (Vigna angularis) is one of the most important legume crops in Asian countries like China, Japan and Korea due to its nutritious protein and starch contents. In spite of its economic importance, gene expression analysis system for gene function verification of adzuki bean is still absent. Therefore, reference genes for gene expression analysis based on the quantitative real time PCR...
متن کاملSelection and Validation of Reference Genes for Gene Expression Analysis in Switchgrass (Panicum virgatum) Using Quantitative Real-Time RT-PCR
Switchgrass (Panicum virgatum) has received a lot of attention as a forage and bioenergy crop during the past few years. Gene expression studies are in progress to improve new traits and develop new cultivars. Quantitative real time PCR (qRT-PCR) has emerged as an important technique to study gene expression analysis. For accurate and reliable results, normalization of data with reference genes...
متن کاملValidation of Reference Genes for Quantitative Expression Analysis by Real-Time RT-PCR in Four Lepidopteran Insects
Quantitative real-time polymerase chain reaction (qPCR) is an efficient and widely used technique to monitor gene expression. Housekeeping genes (HKGs) are often empirically selected as the reference genes for data normalization. However, the suitability of HKGs used as the reference genes has been seldom validated. Here, six HKGs were chosen (actin A3, actin A1, GAPDH, G3PDH, E2F, rp49) in fou...
متن کاملValidation of putative housekeeping genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR
Validation of putative housekeeping genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR. BMC Cancer 2008, 8:350 doi:10.1186/1471-2407-8-350 Vito R Cicinnati ([email protected]) Qingli Shen ([email protected]) Georgios C Sotiropoulos ([email protected]) Arnold Radtke ([email protected]) Guido Gerken ([email protected]...
متن کاملSelection of reference genes for gene expression studies in rat oligodendrocytes using quantitative real time PCR.
Quantitative real time polymerase chain reaction (qPCR) has become a widely used tool to examine gene expression levels. Reliable quantification, however, depends on a proper normalization strategy. Normalization with multiple reference genes is becoming the standard, although the most suitable reference genes depend on the applied treatment as well as the tissue or cell type studied. In this s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Apidologie
سال: 2008
ISSN: 0044-8435,1297-9678
DOI: 10.1051/apido:2008015